post lyophilization Search Results


93
Genovis Inc display report method 20120327 esi pos high
Display Report Method 20120327 Esi Pos High, supplied by Genovis Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mucpharm Pty Ltd bromelain
Bromelain, supplied by Mucpharm Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Labconco post culture liquid
Post Culture Liquid, supplied by Labconco, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+lyophilization/FreeZone+Freeze+Dryer/pm41828815-222-9-13
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91
Revvity erbb2 rnai
Specific tyrphostin <t>ErbB2</t> inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.
Erbb2 Rnai, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+lyophilization/ErbB2+%2F+HER2+(human)%2C+lyophilized/pmc02788648-77-3-13
Average 91 stars, based on 1 article reviews
erbb2 rnai - by Bioz Stars, 2026-10
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Malvern Panalytical particle size
Specific tyrphostin <t>ErbB2</t> inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.
Particle Size, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+lyophilization/Zetasizer+Advance/pmc05636194-64-9-18
Average 99 stars, based on 1 article reviews
particle size - by Bioz Stars, 2026-10
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90
Mediwound Germany lyophilised injectable bromelain-based enzyme
Specific tyrphostin <t>ErbB2</t> inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.
Lyophilised Injectable Bromelain Based Enzyme, supplied by Mediwound Germany, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+lyophilization/lyophilised+injectable+bromelain+based+enzyme/pmc04921045-33-2-4
Average 90 stars, based on 1 article reviews
lyophilised injectable bromelain-based enzyme - by Bioz Stars, 2026-10
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90
Lallemand inc malolactic bacteria oenococcus oeni pn4 mbr
Specific tyrphostin <t>ErbB2</t> inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.
Malolactic Bacteria Oenococcus Oeni Pn4 Mbr, supplied by Lallemand inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/post+lyophilization/oenococcus+oeni+pn4/pm29580483-39-32-43
Average 90 stars, based on 1 article reviews
malolactic bacteria oenococcus oeni pn4 mbr - by Bioz Stars, 2026-10
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Human Adipose Derived Stem Cell Conditioned media, lyophilized Sterile Filtered and tested with Human Adipose Derived Stem Cells in Culture. This product is Serum Free and Animal component free media, for bioprocessing needs. The product
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Mouse Periostin [OSF-2] Recombinant Protein DDDDK Tag Lyophilized from Innovative Research has been recombinantly produced in CHO cells. This is a Lyophilized protein buffered in Contains PBS. Reconstitute with 100 and#956;l sterile water. with a
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Human IgG positive control is the lyophilized powder of human IgG positive control and buffer salts. IgG is a purified antigen.
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POSTN Recombinant Protein N-His Tag Lyophilized from Innovative Research is a recombinant protein lyophilized from sterile 20mm tris,1mm edta, ph 8.0. This preparation has a purity of >85 % as determined by reducing SDS-PAGE. This
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Specific tyrphostin ErbB2 inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tyrphostin ErbB2 Inhibitors AG825 and AG879 Have Non-specific Suppressive Effects on gp130/ STAT3 Signaling

doi: 10.4196/kjpp.2008.12.5.281

Figure Lengend Snippet: Specific tyrphostin ErbB2 inhibitors block IL-6-induced tyrosine phosphorylation of STAT3. (A, B) The effects of an ErbB1 inhibitor (AG1478) or ErbB2 inhibitors (AG825, AG879) on IL-6-induced tyrosine phosphorylation of STAT3 in RT4 cells were analyzed. ErbB2 inhibitors dose-dependently reduced tyrosine phosphorylation of STAT3 induced by IL-6. (C) STAT3 DNA binding activity was analyzed using a STAT3-interacting oligonucleotide conjugated to agarose beads, after 20 min of IL-6 treatment. STAT3 was immunoprecipitated and analyzed via immunoblotting, with antibodies against pSTAT3 or STAT3. (D) An ErbB1/ErbB2 dual inhibitor, GW583340, did not block IL-6-induced tyrosine phosphorylation of STAT3.

Article Snippet: Transient transfection of ErbB2 RNAi was performed with the Dharma FECT transfection reagent (Dharmacon Research), according to the accompanying protocol.

Techniques: Blocking Assay, Phospho-proteomics, Binding Assay, Activity Assay, Immunoprecipitation, Western Blot

Effects of ErbB1 and ErbB2 inhibitors on IL-6-induced tyrosine phosphorylation of STAT3 in primary Schwann cells. (A) The selective inhibition of IL-6-induced (20 min) tyrosine phosphorylation of STAT3 by AG825 was demonstrated in primary Schwann cells. (B) Dose-dependent curves showing the effect of AG825 on RT4 (■) cells and primary Schwann cells (●). The intensity of IL-6-stimulated pSTAT3 band in the absence of AG825 was set as 100% of relative intensity. The curve shows that primary Schwann cells were more sensitive to AG825 than RT4 cells.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tyrphostin ErbB2 Inhibitors AG825 and AG879 Have Non-specific Suppressive Effects on gp130/ STAT3 Signaling

doi: 10.4196/kjpp.2008.12.5.281

Figure Lengend Snippet: Effects of ErbB1 and ErbB2 inhibitors on IL-6-induced tyrosine phosphorylation of STAT3 in primary Schwann cells. (A) The selective inhibition of IL-6-induced (20 min) tyrosine phosphorylation of STAT3 by AG825 was demonstrated in primary Schwann cells. (B) Dose-dependent curves showing the effect of AG825 on RT4 (■) cells and primary Schwann cells (●). The intensity of IL-6-stimulated pSTAT3 band in the absence of AG825 was set as 100% of relative intensity. The curve shows that primary Schwann cells were more sensitive to AG825 than RT4 cells.

Article Snippet: Transient transfection of ErbB2 RNAi was performed with the Dharma FECT transfection reagent (Dharmacon Research), according to the accompanying protocol.

Techniques: Phospho-proteomics, Inhibition

IL-6 does not transactivate ErbB2 in Schwann cells. (A) Western blot analysis with anti-phospho-ErbB2 antibodies showed that IL-6 did not induce tyrosine phosphorylation of ErbB2 at tyrosines 877, 1221 or 1222, whereas NRG induced strong tyrosine phosphorylation of ErbB2 at tyrosine 877 and mild phosphorylation at tyrosines 1221 and 1222. (B) Cell lysates were subjected to immunoprecipitations using either an anti-gp130 antibody or an anti-ErbB2 antibody, and then immunoblotting was performed using an anti-phosphotyrosine antibody (4G10, pY). IL-6 did not induce tyrosine phosphorylation of ErbB2, while it did stimulate the tyrosine phosphorylation of gp130. (C) Cell lysates were subjected to immunoprecipitations using either an anti-gp130 antibody or an anti-IL-6R antibody, and then immunoblotting was performed using an antibody to ErbB2 to demonstrate interactions between gp130 and ErbB2 or between IL-6R and ErbB2. IL-6 induced an interaction between IL-6R and gp130, but did not induce an interaction between ErbB2 and gp130 or IL-6R.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tyrphostin ErbB2 Inhibitors AG825 and AG879 Have Non-specific Suppressive Effects on gp130/ STAT3 Signaling

doi: 10.4196/kjpp.2008.12.5.281

Figure Lengend Snippet: IL-6 does not transactivate ErbB2 in Schwann cells. (A) Western blot analysis with anti-phospho-ErbB2 antibodies showed that IL-6 did not induce tyrosine phosphorylation of ErbB2 at tyrosines 877, 1221 or 1222, whereas NRG induced strong tyrosine phosphorylation of ErbB2 at tyrosine 877 and mild phosphorylation at tyrosines 1221 and 1222. (B) Cell lysates were subjected to immunoprecipitations using either an anti-gp130 antibody or an anti-ErbB2 antibody, and then immunoblotting was performed using an anti-phosphotyrosine antibody (4G10, pY). IL-6 did not induce tyrosine phosphorylation of ErbB2, while it did stimulate the tyrosine phosphorylation of gp130. (C) Cell lysates were subjected to immunoprecipitations using either an anti-gp130 antibody or an anti-IL-6R antibody, and then immunoblotting was performed using an antibody to ErbB2 to demonstrate interactions between gp130 and ErbB2 or between IL-6R and ErbB2. IL-6 induced an interaction between IL-6R and gp130, but did not induce an interaction between ErbB2 and gp130 or IL-6R.

Article Snippet: Transient transfection of ErbB2 RNAi was performed with the Dharma FECT transfection reagent (Dharmacon Research), according to the accompanying protocol.

Techniques: Western Blot, Phospho-proteomics

Specific ablation of ErbB2 function does not inhibit IL-6-induced tyrosine phosphorylation of STAT3. (A) Cells were transfected with control siRNA or ErbB2 siRNA, and IL-6-induced tyrosine phosphorylation of STAT3 was analyzed using Western blot analysis. The suppression of ErbB2 expression with a specific RNAi did not affect the IL-6-induced tyrosine phosphorylation of STAT3. (B) Cells were transfected with an ErbB2 mutant lacking the intracellular domain (ErbB2TR) or empty vector. Two days after transfection, cells were incubated with IL-6 for 20 min, fixed and double-immunostained with an anti-Myc antibody and an anti-pSTAT3 antibody. (C) Quantitative analysis was performed by calculating the percentage of pSTAT3-positive nuclei among Myc-positive cells. Means±S.E from three independent experiments are shown.

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tyrphostin ErbB2 Inhibitors AG825 and AG879 Have Non-specific Suppressive Effects on gp130/ STAT3 Signaling

doi: 10.4196/kjpp.2008.12.5.281

Figure Lengend Snippet: Specific ablation of ErbB2 function does not inhibit IL-6-induced tyrosine phosphorylation of STAT3. (A) Cells were transfected with control siRNA or ErbB2 siRNA, and IL-6-induced tyrosine phosphorylation of STAT3 was analyzed using Western blot analysis. The suppression of ErbB2 expression with a specific RNAi did not affect the IL-6-induced tyrosine phosphorylation of STAT3. (B) Cells were transfected with an ErbB2 mutant lacking the intracellular domain (ErbB2TR) or empty vector. Two days after transfection, cells were incubated with IL-6 for 20 min, fixed and double-immunostained with an anti-Myc antibody and an anti-pSTAT3 antibody. (C) Quantitative analysis was performed by calculating the percentage of pSTAT3-positive nuclei among Myc-positive cells. Means±S.E from three independent experiments are shown.

Article Snippet: Transient transfection of ErbB2 RNAi was performed with the Dharma FECT transfection reagent (Dharmacon Research), according to the accompanying protocol.

Techniques: Phospho-proteomics, Transfection, Control, Western Blot, Expressing, Mutagenesis, Plasmid Preparation, Incubation